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Santa Cruz Biotechnology pvdf membranes
Pvdf Membranes, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotto/Blotto%2C+non-fat+dry+milk/pm42836692-101-2-17
Average 94 stars, based on 238 article reviews

pvdf membranes - by Bioz Stars, 2026-11

94/100 stars

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Incubation:

Article Title: Pre- and postsynaptic upregulation of FasII synergistically underlies neuropathological and behavioral phenotypes in a Drosophila model of myotonic dystrophy.
Article Snippet: The membranes were carefully removed from the Bio-Dot® SF Apparatus and stained with ATX Ponceau S red solution (SigmaAldrich, 09189-1L-F) for total protein quantification. .. Membranes were blocked in 5% Blotto (Santa Cruz Biotech, sc-2324) diluted in 1X TBS-T (10 mM Tris-HCl, 0.15 M NaCl, 0.05% Tween 20) for 1 hour at room temperature, and incubated overnight at 4oC with primary anti-NCAM1 antibody (GeneTex, GTX111684) diluted 1:10,000 in 5% Blotto. .. After three washes in 1X TBS-T, membranes were incubated with secondary HRP-conjugated goat anti-rabbit IgG (Life Technologies, 31460) diluted 1:5,000 in 5% Blotto, for 1h at room temperature.

Article Title: Neuroprotection by Flaxseed Oil in a Model of Hippocampal Injury Induced by Trimethyltin Involves Purinergic System Modulation.
Article Snippet: Briefly, equivalent amounts (20 μg of total proteins) were resolved by SDS-PAGE (4–8%) and transferred onto PVDF support membranes (0.45 mm, Millipore, Darmstadt, Germany). .. After washing in TBST (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.05% Tween 20), the membranes were blocked in 5% Blotto (Santa Cruz Biotechnology, Inc., Dallas, Texas, USA) in TBST for 1 h. The membranes were incubated with guinea pig anti-rat NTPDase1 antibodies (1:1000 dilution; ab66215, Abcam, Cambridge, UK), rabbit anti-rat CD73 (1:2000 dilution; mAb#13160, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-rat ADA (1:1000 dilution: E-AB-66162, Elabsciences, Houston, TX, USA), and rabbit anti-rat ENT1 (1:1000 dilution; 29862-1-AP, Proteintech, Planegg-Martinsried, Germany) overnight at 4 ◦C. ..

Article Title: Pre- and postsynaptic upregulation of FasII synergistically underlies neuropathological and behavioral phenotypes in a Drosophila model of myotonic dystrophy
Article Snippet: The membranes were carefully removed from the Bio-Dot® SF Apparatus and stained with ATX Ponceau S red solution (Sigma-Aldrich, 09189-1L-F) for total protein quantification. .. Membranes were blocked in 5% Blotto (Santa Cruz Biotech, sc-2324) diluted in 1× TBS-T (10 mM Tris-HCl, 0.15 M NaCl, 0.05% Tween 20) for 1 h at room temperature, and incubated overnight at 4 °C with primary anti-NCAM1 antibody (GeneTex, GTX111684) diluted 1:10,000 in 5% Blotto. .. After three washes in 1× TBS-T, membranes were incubated with secondary HRP-conjugated goat anti-rabbit IgG (Life Technologies, 31460) diluted 1:5000 in 5% Blotto, for 1 h at room temperature.

Article Title: Neuroprotection by Flaxseed Oil in a Model of Hippocampal Injury Induced by Trimethyltin Involves Purinergic System Modulation
Article Snippet: Briefly, equivalent amounts (20 μg of total proteins) were resolved by SDS-PAGE (4–8%) and transferred onto PVDF support membranes (0.45 mm, Millipore, Darmstadt, Germany). .. After washing in TBST (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.05% Tween 20), the membranes were blocked in 5% Blotto (Santa Cruz Biotechnology, Inc., Dallas, Texas, USA) in TBST for 1 h. The membranes were incubated with guinea pig anti-rat NTPDase1 antibodies (1:1000 dilution; ab66215, Abcam, Cambridge, UK), rabbit anti-rat CD73 (1:2000 dilution; mAb#13160, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-rat ADA (1:1000 dilution: E-AB-66162, Elabsciences, Houston, TX, USA), and rabbit anti-rat ENT1 (1:1000 dilution; 29862-1-AP, Proteintech, Planegg-Martinsried, Germany) overnight at 4 °C. ..

Article Title: “Chronic morphine treatment induces a conserved Smchd1-dependent epigenetic memory that disrupts X-chromosome inactivation and genomic imprinting”
Article Snippet: To verify equal protein loading and normalize signal intensity, membranes were reproved with a monoclonal mouse anti-beta actin peroxidase antibody (A3854, Sigma) at a 1:25,000 dilution and a polyclonal rabbit anti-H3 antibody (Ab1791, Abcam) at a dilution 1:5000. .. After three washes (5 minutes each) in Blotto, membranes were incubated for 1 hour at room temperature with an HRP-conjugated goat anti-rabbit secondary antibody (Santa Cruz Biotechnology, sc-2004) diluted 1:1000 in Blotto containing 5% BSA. .. Protein bands were visualized using enhanced chemiluminescence on a ChemiDoc XRS imaging system (Bio-Rad).

Western Blot:

Article Title: Inhibition of Bone Morphogenetic Protein Signaling Prevents Tau Pathology in iPSC Derived Neurons and PS19 Mice
Article Snippet: The membranes were blocked in 5% BSA for pSMAD1/5/8 (Millipore, AB3848), BMP4 (Origene, UM500038), and Noggin (R&D Systems, AF719‐SP). .. For all other immunoblots, membranes were blocked in 5% Blotto (Santa Cruz, 2325) in Tris‐buffered saline (Bio‐Rad Laboratories, 1706435). .. Primary antibodies were rabbit anti‐APOE (Cell Signaling Technology, 7074S), mouse anti‐total tau (HT7; Thermo Fisher Scientific, MN1000), mouse anti‐ptau (AT8; Thermo Fisher Scientific, MN1020), rabbit anti‐LC3 (Sigma‐Aldrich, L8918), rabbit anti‐P38 (Cell Signaling Technology, 9211S), rabbit anti‐phospho‐P38 (Cell Signaling Technology, 9211S), and mouse anti‐glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH; Santa Cruz, sc‐365,062).

Saline:

Cell-Signaling:

Article Title: Targeting Artemis sensitizes acute lymphoblastic leukemia cells to topoisomerase 2 poisons by disrupting DNA-PKcs-dependent repair
Article Snippet: .. Antibodies used for theWestern blots were: Artemis (D708V) (1:1000 in 5% BSA/TBST) (Cell Signaling, Cat# 13381), V5-tag (D3H8Q) (1:1000 in BLOTTO) (Cell Signaling, Cat# 13202), phosphoCHK1 (Ser345) (1:1000 in BLOTTO) (Santa Cruz Biotechnology, Cat# sc8408), CHK1 (G-4) (1:1000 in BLOTTO) (Cell Signaling, Cat# 2348), TTRAP (TDP2) (1:5000 in BLOTTO) (Proteintech, Cat# 12203-1-AP), Mouse IgG (H+L) (1:3000 in BLOTTO) (Thermo Fisher, Cat# 31430), and Rabbit IgG (1:10,000 in BLOTTO) (Thermo Fisher, Cat# 12-348). ..

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